Intracellular Localization of DNA Polymerases in the Oocyte of Starfish, Asterina pectinifera. (starfish/DNA polymerase alpha/DNA polymerase beta/germinal vesicle/enucleation)
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چکیده
منابع مشابه
Response of the cortex to the mitotic apparatus during polar body formation in the starfish oocyte of Asterina pectinifera.
In order to understand the mechanism of unequal division, polar body formation was investigated using the oocytes of the starfish, Asterina pectinifera. Cortical actin filaments were quantitatively measured after staining the maturing oocytes with fluorescently labeled phalloidin using a computer and image-processing software. Before polar body formation, at first the actin filaments at the ani...
متن کاملA relaxin-like peptide purified from radial nerves induces oocyte maturation and ovulation in the starfish, Asterina pectinifera.
Gonad-stimulating substance (GSS) of starfish is the only known invertebrate peptide hormone responsible for final gamete maturation, rendering it functionally analogous to the vertebrate luteinizing hormone (LH). Here, we purified GSS of starfish, Asterina pectinifera, from radial nerves and determined its amino acid sequence. The purified GSS was a heterodimer composed of 2 different peptides...
متن کاملIsolation and characteristics of trypsin from pyloric ceca of the starfish Asterina pectinifera.
Trypsin was purified from pyloric ceca of the starfish Asterina Pectinifera by ammonium sulfate precipitation, gel filtration, and cation-exchange chromatography. Final enzyme preparation was nearly homogeneous in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and its molecular weight was estimated as approximately 28000. Optimum pH and temperature of A. pectinifera trypsin for hydro...
متن کاملNuclear distribution of proliferating cell nuclear antigen (PCNA) in fertilized eggs of the starfish Asterina pectinifera.
Previous studies (Nomura et al. (1991) Dev. Biol. 143, 289-296 (1993) Dev. Biol. 159, 288-297) determined the time of DNA replication period (S phase) in starfish eggs fertilized either during or after oocyte maturation. Here proliferating cell nuclear antigen (PCNA) localized within nuclei of starfish eggs was detected with an anti-PCNA human antiserum. Using a confocal laser scanning microsco...
متن کاملEstablishment of embryonic axes in larvae of the starfish, Asterina pectinifera.
In order to clarify the relationships between the first cleavage plane and the embryonic axes, early cleavage pattern of the fertilized eggs of the starfish. Asterina pectinifera was reexamined. It was ascertained that the polar bodies were formed at the site to which the germinal vesicle had closely located before the initiation of the meiotic division, and that the first cleavage plane passed...
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ژورنال
عنوان ژورنال: Development, Growth and Differentiation
سال: 1983
ISSN: 0012-1592,1440-169X
DOI: 10.1111/j.1440-169x.1983.00547.x